Part:BBa_K873000

heat shock 10kDa protein(hsp 10) groES coding resquence

GroES: encoding the HSP10（10KDa）, the co-chaperone of groEL, and the two parts compose the groEL/groES system which assist the non-covalent folding or unfolding and the assembly or disassembly of other macromolecular structures .

Results

　　The OD600 of one type of bacteria while time elapses.

                   
We can see from the figure clearly,the bacteria who caring this gene lives a better life in 42℃ than the one without.

For more information on how this part operates in our system, please visit [http://2012.igem.org/Team:SEU_A SEU_A Main page].

Reference:

1. DEBORAH W. COWING*, JAMES C. A. BARDWELLt( May 1985) Consensus sequence for Escherichia coli heat shock gene promoters Biochemistry Proc. Nati. Acad. Sci. USA Vol. 82, pp. 2679-2683. 2. Yang Wang and Pieter L. deHaseth* 2003 Sigma 32-Dependent Promoter Activity In Vivo: Sequence Determinants of the groE Promoter JOURNAL OF BACTERIOLOGY, Oct. 2003, Vol. 185, No. 19 p. 5800–5806. 3. young-Mo Koo, Virgil A. Rhodius, Gen Nonaka, et al. 2009 Reduced capacity of alternative ss to melt promoters ensures stringent promoter recognition Genes Dev. 2009 23: 2426-2436 4. Vivekanandan Aravindhan1, Aparna J. Christy 2009 Mycobacterium tuberculosis groE promoter controls the expression of the bicistronic groESL1 operon and shows differential regulation under stress conditions FEMS Microbiology Letters Volume 292, Issue 1, pages 42–49, March 2009

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NotI site found at 91
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]